White adipose tissue (mouse)
Movie 1: White adipose tissue – extracellular matrix network
Tissue/pathology:
A piece of inguinal (subcutaneous) white adipose tissue (WAT) from a control (non-obese) young adult male C57BL/6J mouse
Antibodies:
Collagen VI.
Movie description:
We see the collagen network around the blood vessels and even around individual adipocytes. Since the procedure removed lipids from the tissue, adipocytes are seen as ’empty holes’ within the fine collagen network. Collagen VI. is the main component of the WAT extracellular matrix.
Download high. res. video 请登录哔哩哔哩观看。Movie 2: Collagen VI. – positive extracellular matrix network and F4/80 – positive macrophages
Tissue/pathology:
A piece of inguinal (subcutaneous) white adipose tissue (WAT) from a control (non-obese) young adult male C57BL/6J mouse
Antibodies:
Collagen VI. (green)
F4/80 (red)
Movie description:
Macrophages are key components in the WAT immune system, which are activated in obesity.
Download high. res. video 请登录哔哩哔哩观看。Movie 3: Collagen VI.- positive extracellular matrix network and F4/80 – positive macrophages; 2D going through
Tissue/pathology:
A piece of inguinal (subcutaneous) white adipose tissue (WAT) from a control (non-obese) young adult male C57BL/6J mouse
Antibodies:
Collagen VI. (green)
F4/80 (red)
Movie description:
Same scan as in MOVIE 2, but now as a 2D serial. The vessels and the shape of the adipocytes (as ‘holes’ with collagen VI+ network around) are seen.
Download high. res. video 请登录哔哩哔哩观看。Movie 4: White adipose tissue noradrenergic (sympathetic) nerves
Tissue/pathology:
A piece of inguinal (subcutaneous) white adipose tissue (WAT) from a control (non-obese) young adult male C57BL/6J mouse
Antibodies:
Tyrosine hydroxylase
Movie description:
This movie shows the mouse WAT sympathetic innervation in 3D. Note the surprisingly dense innervation that follows blood vessels. Note the fine fibres even around individual adipocytes.
Download high. res. video 请登录哔哩哔哩观看。Tissue: Dr. Csaba Adori and Dr. Jan Mulder; iDISCO reaction, light sheet microscopy, postprocess and videos: Dr. Csaba Adori