Inner ear (mouse)
Movie 1: Cochlea
Tissue/pathology:
Albino mouse, P5.
Antibodies:
Calretinin
Movie description:
The hair cells, the spiral ganglion cell bodies and processes (dendrites to hair cells and axons to central projection) are shown. After 360o rotation, zoom to the axons that project to the brainstem cochlear nucleus. These structures are involved in the detection and transmission of acoustic signals.
Download high. res. video 请登录哔哩哔哩观看。Movie 2: Cochlear and vestibular system I.
Tissue/pathology:
C57BL/6J mouse, P3.
Antibodies:
Calretinin
Movie description:
The hair cells and the spiral ganglion are seen. We can also see the shape of the Reissner membrane (as autofluorescence shadow). The vestibular hair cells and a subpopulation of vestibular nucleus neurons are also depicted. Note the abundant dendrites that reach the vestibular hair cell populations. The vestibular system is involved in the detection and transmission of information concerning the balance and spatial position of the body (head). After 360o rotation, zoom to the vestibular system: to the vestibular hair cells (located in the three semicircular canals), to the vestibular ganglion dendrites and to sparse vestibular ganglion cell bodies.
Download high. res. video 请登录哔哩哔哩观看。Movie 3: Cochlear and vestibular system II.
Tissue/pathology:
C57BL/6J mouse, P3.
Antibodies:
Myo7A (green)
Parvalbumin (red)
Movie description:
This movie depicts elements of the cochlear and vestibular systems in a P3 B6 mouse inner ear, using Myo7A as immuno-marker for hair cells (green) and parvalbumin as immuno-marker for neuronal cell bodies and some of their processes (red). During continuous rotation and shifting between channels, we see all the hair cell populations (cochlear hair cells as a ‘snake’-like structure and the vestibular hair cells as horse-shoes or little paws) as well as the spiral and vestibular ganglia neuronal cell bodies. Notably, while the cochlear hair cells are immuno-labelled also for parvalbumin, the vestibular hair cells are positive only for Myo7A. This reflects the (neuro)chemical heterogeneity of these cell populations.
Download high. res. video 请登录哔哩哔哩观看。Movie 4: Close up to hair cells and spiral ganglion
Tissue/pathology:
C57BL/6J mouse, P3.
Antibodies:
Calretinin
Movie description:
The rotations show the shape of the structure of the hair cells and spiral ganglion cells from more perspectives. Then, zoom to the hair cells / spiral ganglion processes / spiral ganglion neurons at the basis of the cochlea and, as on a ladder, the picture slowly moves upper and upper towards the apex of the cochlea. This spatial organization of hair cells in the Corti-organ is important in the discrimination of lower and higher frequency voices. Then, more zoom to the apical inner hair cells.
Download high. res. video 请登录哔哩哔哩观看。Movie 5: Close up to cochlear inner and outer hair cells.
Tissue/pathology:
C57BL/6J mouse P3.
Antibodies:
Myo7A (green)
Parvalbumin (red)
Movie description:
This movie shows the cochlear hair cells in higher magnification. After some rotations, the zoom to hair cells ca. in the middle of the of the Corti-organ. Then, the channel shifts only to Myo7A (green) and the inner- and outer hair cells are shown from various perspectives. The inner hair cells (stronger staining, single layer) detect and transform the sound vibrations to electric signal in the cochlear fluid. The outer hair cells (four layers, weaker staining, behind the inner hair cell layer) mechanically amplify the lower level sounds.
Download high. res. video 请登录哔哩哔哩观看。Tissue: Dr. Francois Lallemend and Dr. Charles Petitpre; iDISCO reaction, light sheet microscopy, postprocess and videos: Dr. Csaba Adori