Breast cancer impacts over 2 million women in the world every year; it is the second most frequent cause of cancer-related death among women. These movies show the three-dimensional ductular structure and vasculature of various breast tumours compared to the healthy breast tissue.
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Movie 1: Breast ductular structure in healthy young woman
Tissue/pathology:
Healthy young woman breast tissue (25 y old)
Antibodies:
e-cadherin
Movie description:
360ohorizontal and vertical turn of a 3D crop, showing multiple ducts with the epithelial marker e-cadherin.
Movie 4: Overview scan to demonstrate healthy and tumorous ducts in ductal carcinoma
Tissue/pathology:
Breast tissue containing ductal carcinoma in situ
Antibodies:
e-cadherin (with surface creation)
Movie description:
360ohorizontal rotation, then segmentation of a single healthy tube with a large tumour outgrowth. Rotation of the tumour, then demonstration of the shape of tumour with 3D surface creation. Then, zoom to the tumour till single cell resolution in maximum intensity projection (MIP) mode again.
360ohorizontal and vertical turn of a 3D crop, demonstrating invasive tumour. This tissue scan suffers from some technical problems like limited ab penetration due to suboptimal fixation and dot artefacts due to calcification deposits in the tissue.
Movie 6: Normal vasculature of healthy breast tissue
Tissue/pathology:
Healthy breast tissue
Antibodies:
CD34
Movie description:
High resolution zoom to normal CD34+ vasculature of a healthy piece of tissue. Exploring vessels with close-up. CD34 = endothelial marker, depicting all blood vessels in the sample.
