Gut and its nervous system (mouse and human)

These videos show the histological structure of the human colon and the mouse small intestine as well as several elements of the gut nervous system in human and mouse in three-dimension.

Movie 1: Human colon, structure and innervation as 2D going-through

Tissue/pathology:

Human colon

Antibodies:

488 autofluorescence (brownish-red)
PGP9.5 (gray)

Movie description:

Autofluorescence (AF) is brownish-red, innervation is bluish gray. AF depicts the structure of a piece of human colon (bit damaged and distorted due to sampling and processing). PGP9.5 is a pan-neuronal marker and depicts all neuronal elements (bluish gray), so here we basically see the full innervation (including sympathetic, parasympathetic elements and the full enteral nervous system). Note the heavy fine innervation of the intestinal crypts throughout.

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Movie 2: Same acquisition as MOVIE 1, but now in 3D

Tissue/pathology:

Human colon

Antibodies:

488 autofluorescence (brownish-red)
PGP9.5 (gray)

Movie description:

First, we see the autofluorescence (brownish) that depicts the shape of the organ, together with the PGP9.5 (all neuronal elements, bluish gray). After some turn, zoom to the nerves, first to the dense fine innervation of the crypts and then to the thicker nerves and neurons of the gut wall, in various perspectives.

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Movie 3: A piece of human colon with the epithelial marker e-cadherin

Tissue/pathology:

Human colon

Antibodies:

e-cadherin

Movie description:

After some turn, zoom to the intestinal crypts. E-cadherin, as an epithelial marker, depicts the shape of the crypts or villi.

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Movie 4: Same as MOVIE 3 but with blend 3D rendering mode

Tissue/pathology:

Human colon

Antibodies:

e-cadherin

Movie description:

This is a bit ‘artificial’ but provides a better depiction of the shape of the structure. More zoom to the crypts.

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Movie 5: Small intestine stained with the epithelial marker e-cadherin (green) and the smooth muscle marker SMA (smooth muscle actin, red)

Tissue/pathology:

Young adult male C57BL/6J control mouse small intestine

Antibodies:

e-cadherin (green)
smooth muscle actin (SMA, red)

Movie description:

After some turns with both channels, we see a close up to the intestinal villi (depicted by e-cadherin), and then to the muscle of the intestine. Note the robust circular muscle around the intestine and the finer muscle fibers in the axis of the villi.

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Movie 6: Blend 3D rendering of a piece of mouse small intestine, based on autofluorescence

Tissue/pathology:

Young adult male C57BL/6J control mouse small intestine

Antibodies:

488 autofluorescence

Movie description:

depicting the structure. Note the villi inside the lumen.

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Movie 7: Mouse small intestine, sympathetic (noradrenergic) nerves

Tissue/pathology:

Young adult male C57BL/6J control mouse small intestine

Antibodies:

488 autofluorescence (brownish)
Tyrosine hydroxylase (bluish-gray)

Movie description:

First, we see the autofluorescence channel (brownish) depicting the structure (same as in MOVIE 6, but in MIP). Then, switch to the TH channel, demonstrating the distribution of the noradrenergic nerves in the gut wall (innervating mainly the outer muscles). Then, switch to the TH channel only and close up to the sympathetic innervations from more perspectives.

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These videos show the histological structure of the human colon and the mouse small intestine as well as several elements of the gut nervous system in human and mouse in three-dimension.

FOR THE BEST EXPERIENCE, RE-ADJUST MANUALLY (⚙) THE STREAMING QUALITY OF YOUTUBE VIDEOS, IF NECESSARY (FROM ‘AUTO’ TO 1080p/1440p, DEPENDING ON YOUR INTERNET CONNECTION).

Movie 1: Human colon, structure and innervation as 2D going-through

Tissue/pathology:

Antibodies:

Movie description:

Movie 2: Same acquisition as MOVIE 1, but now in 3D

Tissue/pathology:

Antibodies:

Movie description:

Movie 3: A piece of human colon with the epithelial marker e-cadherin

Tissue/pathology:

Antibodies:

Movie description:

Movie 4: Same as MOVIE 3 but with blend 3D rendering mode

Tissue/pathology:

Antibodies:

Movie description:

Movie 5: Small intestine stained with the epithelial marker e-cadherin (green) and the smooth muscle marker SMA (smooth muscle actin, red)

Tissue/pathology:

Antibodies:

Movie description:

Movie 6: Blend 3D rendering of a piece of mouse small intestine, based on autofluorescence

Tissue/pathology:

Antibodies:

Movie description:

Movie 7: Mouse small intestine, sympathetic (noradrenergic) nerves

Tissue/pathology:

Antibodies:

Movie description:

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Cholinergic nerves in the muscle (mouse)

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Gut and its nervous system
(mouse and human)

Human substantia nigra
(human)

White adipose tissue
(mouse)

Spleen
(mouse)

Placenta
(human)

Pancreas
(mouse)

Orexin and narcolepsy
(mouse)

Liver – fatty liver
(mouse and human)

Kidneys
(mouse)

Inner ear
(mouse)

Alzheimer’s disease
(human)

Heart and heart sympathetic nerves
(mouse)

GPR151 orphan receptor in the mouse brain
(mouse)

Developing nervous system based on NECAB2 staining
(mouse)

Cholinergic nerves in the muscle
(mouse)

Catecholaminergic neurons in the mouse brain
(mouse)

Breast cancer
(human)

Astroglia around brain blood vessel
(mouse)