Developing nervous system based on NECAB2 staining
(mouse)

Movie 1: Full embryo, 3D overview

Tissue/pathology:

E14 embryo; C57BL/6J mouse

Antibodies:

NECAB2

Movie description:

After some rotation, focus on the (i) caudal spinal region- dorsal root ganglia; (ii) developing hand innervation; (iii) facial nerves, innervating the whiskers; and on the (iv) frontal cortex of the brain

Movie 2: 2D rostro-caudal going through the E15 mouse head scan, coronal orientation

Tissue/pathology:

E15 embryo; C57BL/6J mouse

Antibodies:

NECAB2

Movie description:

This 2D video was prepared based on the same scan that was used for MOVIE 3, so here one can compare how the serial of 2D pictures (MOVIE 2) vs. the entire 3D-reconstructed structure (MOVIE 3) look like.

Movie 3: Mouse E15 head, 3D reconstruction

Tissue/pathology:

E15 embryo; C57BL/6J mouse

Antibodies:

NECAB2

Movie description:

After building up the 3D structure from the 2D planes and some rotations, zoom to the facial nerves, then zoom into the facial skeleton via the pupil and focus more on the trigeminal ganglion and nerve branches. Then, moving up to the brain, again. Frontal lobe neurons and then the ganglionic eminence are seen with neurons migrating towards the cortex.

Unfortunately, some dot-artefacts stuck inside the facial skull bones, which is a bit disturbing, but this is inevitable with this method and the digital removal is difficult due to the heterogeneous immuno-signal.