Breast cancer (human)

Breast cancer impacts over 2 million women in the world every year; it is the second most frequent cause of cancer-related death among women. These movies show the three-dimensional ductular structure and vasculature of various breast tumours compared to the healthy breast tissue.

‘Breast cancer’ short film

Movie 1: Breast ductular structure in healthy young woman

Tissue/pathology:

Healthy young woman breast tissue (25 y old)

Antibodies:

e-cadherin

Movie description:

360^o horizontal and vertical turn of a 3D crop, showing multiple ducts with the epithelial marker e-cadherin.

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Movie 2: Breast ductular structure in mature healthy woman

Tissue/pathology:

Healthy mature woman breast tissue (61 y old)

Antibodies:

e-cadherin

Movie description:

360^o horizontal and vertical turn of a 3D crop, showing a single duct, the remaining adipose tissue is negative for e-cadherin.

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Movie 3: High resolution scan, multiple e-cadherin immunoreactive ducts in fibroadenoma

Tissue/pathology:

Fibroadenoma (benign tumour) breast tissue

Antibodies:

e-cadherin

Movie description:

360^o horizontal rotation, then zoom to demonstrate fine shape of ducts, with the resolution where individual epithelial cells are visible.

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Movie 4: Overview scan to demonstrate healthy and tumorous ducts in ductal carcinoma

Tissue/pathology:

Breast tissue containing ductal carcinoma in situ

Antibodies:

e-cadherin (with surface creation)

Movie description:

360^o horizontal rotation, then segmentation of a single healthy tube with a large tumour outgrowth. Rotation of the tumour, then demonstration of the shape of tumour with 3D surface creation. Then, zoom to the tumour till single cell resolution in maximum intensity projection (MIP) mode again.

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Movie 5: Invasive ductal carcinoma

Tissue/pathology:

Invasive ductal carcinoma

Antibodies:

e-cadherin

Movie description:

360^o horizontal and vertical turn of a 3D crop, demonstrating invasive tumour. This tissue scan suffers from some technical problems like limited ab penetration due to suboptimal fixation and dot artefacts due to calcification deposits in the tissue.

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Movie 6: Normal vasculature of healthy breast tissue

Tissue/pathology:

Healthy breast tissue

Antibodies:

CD34

Movie description:

High resolution zoom to normal CD34+ vasculature of a healthy piece of tissue. Exploring vessels with close-up. CD34 = endothelial marker, depicting all blood vessels in the sample.

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Movie 7: Vasculature in invasive ductal carcinoma

Tissue/pathology:

Invasive ductal carcinoma

Antibodies:

CD34

Movie description:

High resolution zoom to abnormal CD34+ vasculature of an invasive tumour sample. Exploring vessels with close-up. Limited ab penetration.

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Tissue: Prof. Fredrik Ponten; iDISCO reaction, light sheet microscopy, postprocess and videos: Dr. Csaba Adori

Breast cancer impacts over 2 million women in the world every year; it is the second most frequent cause of cancer-related death among women. These movies show the three-dimensional ductular structure and vasculature of various breast tumours compared to the healthy breast tissue.

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Movie 1: Breast ductular structure in healthy young woman

Tissue/pathology:

Antibodies:

Movie description:

Movie 2: Breast ductular structure in mature healthy woman

Tissue/pathology:

Antibodies:

Movie description:

Movie 3: High resolution scan, multiple e-cadherin immunoreactive ducts in fibroadenoma

Tissue/pathology:

Antibodies:

Movie description:

Movie 4: Overview scan to demonstrate healthy and tumorous ducts in ductal carcinoma

Tissue/pathology:

Antibodies:

Movie description:

Movie 5: Invasive ductal carcinoma

Tissue/pathology:

Antibodies:

Movie description:

Movie 6: Normal vasculature of healthy breast tissue

Tissue/pathology:

Antibodies:

Movie description:

Movie 7: Vasculature in invasive ductal carcinoma

Tissue/pathology:

Antibodies:

Movie description:

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Breast cancer
(human)

Human substantia nigra
(human)

White adipose tissue
(mouse)

Spleen
(mouse)

Placenta
(human)

Pancreas
(mouse)

Orexin and narcolepsy
(mouse)

Liver – fatty liver
(mouse and human)

Kidneys
(mouse)

Inner ear
(mouse)

Alzheimer’s disease
(human)

Heart and heart sympathetic nerves
(mouse)

Gut and its nervous system
(mouse and human)

GPR151 orphan receptor in the mouse brain
(mouse)

Developing nervous system based on NECAB2 staining
(mouse)

Cholinergic nerves in the muscle
(mouse)

Catecholaminergic neurons in the mouse brain
(mouse)

Astroglia around brain blood vessel
(mouse)