The neuropeptide galanin is necessary for the development of specific neuronal subsets.
In this ongoing collaborative project, we have explored the distribution of galanin-expressing neurons in the developing mouse thalamus with volume imaging technology, using anti-RFP volume immunostaining in gal-tdTomato+ mouse line.
Preprogalanin expression (volume immunostaining of galanin-RFP) in the developing ventrobasalis nucleus of the thalamus at P7 in gal-tdTomato+ mouse line. The volume of ventrobasalis thalamic nucleus is indicated as a green surface in B1-2. RFP+ cells are red in B2-3 and are indicated by white dots in B4. (From the PhD thesis of Joanne Bakker).