In this collaborative project, Kazunori Sunadome and Alek Erickson from the laboratory of Igor Adameyko, Karolinska Institutet, Department of Cell and Molecular Biology, investigate how myofibrils and the developing cartilage interact with each other during skeletal muscle development. I contribute to this project with performing light sheet fluorescence microscopy and 3D image reconstruction of early mouse embryos with whole mount fluorescent in situ hybridization.
Light sheet fluorescence microscopy and 3D image reconstruction of early mouse embryos with whole mount fluorescent in situ hybridization for myoblast (green) and cartilage (gray) markers. Whole mount staining: Kazunori Sunadome. Light sheet microscopy imaging: Csaba Adori